Generator

Part:BBa_K265009:Design

Designed by: Timothy Fenton   Group: iGEM09_UC_Davis   (2009-10-04)

Ice Nucleation Protein NC + Proteolytic Cleavage Sequence


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 405
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is in RFC12 format to accommodate fusion proteins.

Source

Both INPNC and the proteolytic cleavage sequence were sequenced by Mr. Gene. INPNC originates from Pseudomonas syringae and the cleavage sequence was taken from the first 37 amino acids in the phaZ1 gene.

References

1. Se Whan Park, Moon Gyu Chung, Hwa Young Lee, Jeong Yoon Kim, and Young Ha Rhee. (2008). Stable expression and secretion of the polyhydroxybutyrate depolymerase of Paucimonas lemoignei in Escherichia coli. J. Microbiology. 46(6). pp. 662-669.