Generator
Part:BBa_K265009:Design
Designed by: Timothy Fenton Group: iGEM09_UC_Davis (2009-10-04)
Ice Nucleation Protein NC + Proteolytic Cleavage Sequence
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 405
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in RFC12 format to accommodate fusion proteins.
Source
Both INPNC and the proteolytic cleavage sequence were sequenced by Mr. Gene. INPNC originates from Pseudomonas syringae and the cleavage sequence was taken from the first 37 amino acids in the phaZ1 gene.
References
1. Se Whan Park, Moon Gyu Chung, Hwa Young Lee, Jeong Yoon Kim, and Young Ha Rhee. (2008). Stable expression and secretion of the polyhydroxybutyrate depolymerase of Paucimonas lemoignei in Escherichia coli. J. Microbiology. 46(6). pp. 662-669.